Search results for "Séquence nucléotidique"

showing 2 items of 2 documents

Cytological and molecular characterization of three gametoclones of Citrus clementina

2013

Abstract Background Three gametoclonal plants of Citrus clementina Hort. ex Tan., cv. Nules, designated ESP, FRA, and ITA (derived from three labs in Spain, France, and Italy, respectively), were selected for cytological and molecular characterization in order to elucidate genomic rearrangements provoked by haploidization. The study included comparisons of their ploidy, homozygosity, genome integrity, and gene dosage, using chromosome counting, flow cytometry, SSR marker genotyping, and array-Comparative Genomic Hybridization (array-CGH). Results Chromosome counting and flow cytometry revealed that ESP and FRA were haploid, but ITA was tri-haploid. Homozygous patterns, represented by a sing…

0106 biological sciencesCitrus[SDV]Life Sciences [q-bio]ÉvolutionPlant ScienceHaploidyHORT EX TAN01 natural sciencesGenomeF30 - Génétique et amélioration des planteshttp://aims.fao.org/aos/agrovoc/c_3185SSRSMARKERShttp://aims.fao.org/aos/agrovoc/c_2091http://aims.fao.org/aos/agrovoc/c_8837Citrus clementinaGynogénèseGenetics0303 health scienceshttp://aims.fao.org/aos/agrovoc/c_1637Homozygotehttp://aims.fao.org/aos/agrovoc/c_27583http://aims.fao.org/aos/agrovoc/c_26859Culture d'anthèreCytologieRECOVERYSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeGENOMEhttp://aims.fao.org/aos/agrovoc/c_3490[SDE]Environmental SciencesGametoclonal variationhttp://aims.fao.org/aos/agrovoc/c_6ce991ddPloidyhttp://aims.fao.org/aos/agrovoc/c_4026Genome PlantResearch ArticleLocus des caractères quantitatifsSéquence nucléotidiqueAnther cultureGamèteLocus (genetics)BiologyGenome sequencingGene dosageAnther culture Gynogenesis Gametoclonal variation Genome sequencingDNA sequencinghttp://aims.fao.org/aos/agrovoc/c_489103 medical and health sciencesGynogenesisRETICULATA BLANCOREGENERATIONHaploïdiehttp://aims.fao.org/aos/agrovoc/c_3081Anther culture;Gynogenesis;Gametoclonal variation;Genome sequencing;HORT EX TAN;ANTHER CULTURE;RETICULATA BLANCO;REGENERATION;RECOVERY;MARKERS;GENOME;SSRS[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyAllelehttp://aims.fao.org/aos/agrovoc/c_37974GeneGenotypingAlleles030304 developmental biologyhttp://aims.fao.org/aos/agrovoc/c_2745Biologie moléculairehttp://aims.fao.org/aos/agrovoc/c_7273010606 plant biology & botany
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Transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pAt megaplasmid in Agrobacterium tumefaciens.

2016

Background Agrobacterium tumefaciens strain P4 is atypical, as the strain is not pathogenic and produces a for this species unusual quorum sensing signal, identified as N-(3-hydroxy-octanoyl)-homoserine lactone (3OH,C8-HSL). Results By sequence analysis and cloning, a functional luxI-like gene, named cinI, has been identified on the At plasmid of A. tumefaciens strain P4. Insertion mutagenesis in the cinI gene and transcriptome analyses permitted the identification of 32 cinI-regulated genes in this strain, most of them encoding proteins responsible for the conjugative transfer of pAtP4. Among these genes were the avhB genes that encode a type 4 secretion system (T4SS) involved in the forma…

0301 basic medicineacylhomoserime lactoneIdentification[SDV]Life Sciences [q-bio]AgrobacteriumPlasmidePlant Rootsfluids and secretionsPlasmidSolanum lycopersicumhttp://aims.fao.org/aos/agrovoc/c_16014Expression des gènesDynamique des populationsCloning MolecularPhylogenyGeneticsbiology000 - Autres thèmeshttp://aims.fao.org/aos/agrovoc/c_27583food and beveragesAgrobacterium tumefaciensLactonehttp://aims.fao.org/aos/agrovoc/c_768[SDV] Life Sciences [q-bio]Quorum sensingT4SSConjugation GeneticPropriété biologiquehttp://aims.fao.org/aos/agrovoc/c_35128PlasmidsResearch Articlehttp://aims.fao.org/aos/agrovoc/c_4145BiotechnologyDtr systemSéquence nucléotidiqueAgrobacteriumSequence analysisMutagenesis (molecular biology technique)At plasmid03 medical and health scienceshttp://aims.fao.org/aos/agrovoc/c_4891Bacterial Proteinsstomatognathic systemhttp://aims.fao.org/aos/agrovoc/c_3081Geneticshttp://aims.fao.org/aos/agrovoc/c_1501Acylhomoserine lactoneTranscriptomicsGenehttp://aims.fao.org/aos/agrovoc/c_6111H20 - Maladies des plantesCloning[ SDV ] Life Sciences [q-bio]Bactériologiehttp://aims.fao.org/aos/agrovoc/c_27444Sequence Analysis RNATranscription géniqueConjugationGene Expression ProfilingBiologie moléculaireGene Expression Regulation Bacterialbiochemical phenomena metabolism and nutritionQuorum sensing;Agrobacterïum;At plasmid;transcriptomics;conjugation;T4SS;Dtr system;Acylhomoserine lactonebiology.organism_classificationhttp://aims.fao.org/aos/agrovoc/c_27527Quorum sensinghttp://aims.fao.org/aos/agrovoc/c_3791030104 developmental biologyAgrobacterium tumefaciensbacteriaGenetic Fitness
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